I-Lateral flow assays (LFAs) zilula ukuzisebenzisa, izixhobo zokuxilonga ezinokulahlwa ezinokuvavanya ii-biomarkers kwiisampulu ezinje ngamathe, igazi, umchamo, kunye nokutya.Iimvavanyo zinenani leenzuzo ngaphezu kobunye ubugcisa bokuxilonga kuquka:
❆ Ubulula: Ubulula bokusebenzisa olu mvavanyo alunakuthelekiswa - yongeza nje amathontsi ambalwa kwisampulu yezibuko kwaye ufunde iziphumo zakho ngeliso kwimizuzu embalwa kamva.
❆ EzoQoqosho: Uvavanyo alubizi kakhulu - ngokuqhelekileyo lungaphantsi kwedola enye ngovavanyo lokwenziwa ngokwesikali.
❆ Omelele: Uvavanyo lunokugcinwa kwiqondo lobushushu le-ambient kwaye lube neminyaka emininzi yobomi.
Iibhiliyoni zemichilo yovavanyo iveliswa minyaka le ukuze kufunyaniswe izifo ezosulela ngesondo, izifo ezithwalwa ziingcongconi, isifo sephepha, ihepatitis, ukukhulelwa kunye novavanyo lokuchuma, iimpawu zentliziyo, uvavanyo lwe-cholesterol/lipid, iziyobisi zokusetyenziswa kakubi, uxilongo lwezilwanyana, kunye nokhuseleko lokutya, phakathi abanye.
I-LFA yenziwe ngesampula yesampuli, i-conjugate pad, i-nitrocellulose strip equlethe imigca yokuvavanya kunye nokulawula, kunye ne-wicking pad.Icandelo ngalinye liwelana ubuncinane nge-1-2 mm eyenza ukuhamba kwe-capillary okungathintekiyo kwisampuli.
Ukusebenzisa isixhobo, isampuli yolwelo njengegazi, i-serum, i-plasma, umchamo, i-saliva, okanye i-solubilized solids, yongezwa ngokuthe ngqo kwi-pad yesampuli kwaye ikhohlakele ngokusebenzisa i-lateral flow device.Isampulu iphedi yenza isampuli ingathathi hlangothi kwaye ihluze iinxalenye ezingafunekiyo ezifana neeseli ezibomvu zegazi.Isampulu emva koko inokuhamba ingathintelwa kwiphedi ye-conjugate equlethe i-nanoparticles enemibala enamandla okanye ene-fluorescent ene-antibody kumphezulu wazo.Xa i-liquid ifikelela kwi-conjugate pad, ezi nanoparticles ezomileyo zikhululwa kwaye zixutywe kunye nesampuli.Ukuba kukho nabaphi na abahlalutyi ekujoliswe kubo kwisampulu ebonwa li-antibody, ezi ziya kubophelela kwi-antibody.I-analyte-bound bound nanoparticles ke ihamba nge-membrane ye-nitrocellulose kunye nomgca omnye okanye ngaphezulu kovavanyo kunye nomgca wokulawula.Umgca wokuvavanya (obhalwe ngo-T kumfanekiso ongentla) yinto ephambili yokufunda ukuxilongwa kwaye iqukethe iiprotheni ezingenakunyakaziswa ezinokubopha i-nanoparticle ukuvelisa umqondiso ohambelana nobukho be-analyte kwisampuli.Ulwelo luqhubeka luhamba ngaphaya komcu de lufike kumgca wolawulo.Umgca wokulawula (obhalwe C kumfanekiso ongentla) uqulethe i-affinity ligands eya kubopha i-nanoparticle conjugate kunye okanye ngaphandle kwe-analyte ekhoyo kwisisombululo sokuqinisekisa ukuba i-assay isebenza ngokufanelekileyo.Emva komgca wolawulo, i-fluid igeleza kwi-pad wicking efunekayo ukuba ithathe yonke isampuli yolwelo ukuqinisekisa ukuba kukho ukuhamba okuhambelanayo kwimigca yokuvavanya kunye nokulawula.Kwezinye iimvavanyo, isithinteli sokuleqa sisetyenziswa kwisampulu yezibuko emva kokwaziswa kwesampulu ukuqinisekisa ukuba yonke isampuli ithuthwa ngapha komcu.Emva kokuba yonke isampuli idlule kwimigca yovavanyo kunye nokulawula, uvavanyo lugqityiwe kwaye umsebenzisi unokufunda iziphumo.
Ixesha lokuhlalutya lixhomekeke kuhlobo lwe-membrane esetyenzisiweyo kwi-lateral flow assay (i-membrane ezinkulu zihamba ngokukhawuleza kodwa ngokuqhelekileyo zincinci) kwaye ngokuqhelekileyo zigqitywe ngaphantsi kwemizuzu eyi-15.
Ixesha lokuposa: Nov-27-2021