Faka iPakeji yePakethe yeSixhobo seHCV ngokukhawuleza
Intshayelelo
I-Hepatitis C Virus (HCV) yintsholongwane encinci, egqunyiweyo, eyakhayo, enemisonto enye ye-RNA.
I-HCV ngoku yaziwa njengoyena nobangela wosulelo lwe-non-A, non-B hepatitis olusasazwa ngumzali.I-antibody kwi-HCV ifunyenwe ngaphezulu kwe-80% yezigulana ezine-non-A, non-B hepatitis ebhalwe kakuhle.Iindlela eziqhelekileyo ziyasilela ukuyibeka yodwa intsholongwane kwinkcubeko yeeseli okanye ukuyibona nge-electron microscope.Ukudibanisa i-genome yentsholongwane kuye kwenza ukuba kube lula ukuphuhlisa iimvavanyo ze-serologic ezisebenzisa i-antigens eziphinda ziphinde zibuyele.Xa kuthelekiswa nesizukulwana sokuqala se-HCV EIA sisebenzisa i-antigen enye ephinda iphinda-phinda, ii-antigens ezininzi ezisebenzisa iprotein ephinda-phindayo kunye/okanye iipeptide zokwenziwa zongezwe kuvavanyo olutsha lwe-serologic ukunqanda ukuphinda-phinda ukuphinda-phinda kusebenze kunye nokwandisa ubuntununtunu bovavanyo lwe-antibody ye-HCV.
Umgaqo
Isixhobo soVavanyo oluKhawulezayo lwe-HCV (iSerum/Plasma) senzelwe ukufumanisa izilwa-buhlungu kwi-HCV ngokutolika okubonakalayo kophuhliso lombala kumgca wangaphakathi.I-membrane yayingenakunyakaziswa kunye neprotheni A kwindawo yokuvavanya.Ngexesha lovavanyo, i-specimen ivunyelwe ukuba isabele nge-antigens enemibala ye-HCV ye-colloidal conjugates yegolide, eyayifakwe ngaphambili kwisampula yovavanyo.Emva koko umxube uhamba kwi-membrane ngesenzo se-capillary, kwaye usebenzisana nama-reagents kwi-membrane.Ukuba bekukho izilwa-buhlungu ezaneleyo ze-HCV kwisampulu, ibhendi enemibala iya kusuka kwindawo yovavanyo yenwebu.Ubukho beli bhanti elinemibala libonisa umphumo omuhle, ngelixa ukungabikho kwawo kubonisa umphumo ombi.Ukubonakala kwebhanti enemibala kwindawo yokulawula isebenza njengolawulo lwenkqubo.Oku kubonisa ukuba umthamo ofanelekileyo we-specimen wongeziwe kwaye i-membrane wicking yenzeke.
Amacandelo eKit
| Izixhobo zovavanyo ezipakishwe ngumntu ngamnye | Isixhobo ngasinye sinomgca onemibala edibanisayo kunye nezixhobo ezisebenzayo ezisasazeke ngaphambili kwimimandla ehambelanayo. |
| Iipayipi ezilahlayo | Ukongeza imizekelo sebenzisa |
| Isithinteli | I-phosphate buffered saline kunye nepreservative |
| Faka iphakheji | Ngomyalelo wokusebenza |
Izinto Eziyimfuneko Kodwa Ezingabonelelwanga
| Isingxobo sokuqokelelwa kwesampuli | Ukuze kusetyenziswe imizekelo yokuqokelela |
| Isibali-xesha | Ukusetyenziswa kwexesha |
| Centrifuge | Ukulungiselela imizekelo ecacileyo |
Ukulumkela
★ Ukusetyenziswa koxilongo lwe-in vitro kuphela.
★ Ungasebenzisi emva komhla wokuphelelwa oboniswe kwiphakheji.Musa ukulusebenzisa uvavanyo ukuba isingxobo sefoyile saso sonakalisiwe.Musa ukuphinda usebenzise iimvavanyo.
★ Le khithi iqulethe iimveliso zemvelaphi yezilwanyana.Ulwazi oluqinisekisiweyo lwemvelaphi kunye / okanye ubume bococeko lwezilwanyana aluqinisekisi ngokupheleleyo ukungabikho kwee-agent ze-pathogenic ezihambisayo.Kuyacetyiswa ke ngoko ukuba ezi mveliso ziphathwe njengenokosulela, kwaye ziphathwe ngokuqwalasela imiqathango yokhuseleko yesiqhelo (ungagilisi okanye ukusezele umoya).
• Kuphephe ukungcoliseka ngokunqamlezayo kwesampulu ngokusebenzisa isikhongozeli esitsha sokuqokelelwa kwesampulu kumzekelo ngamnye ofunyenweyo.
★ Funda inkqubo yonke ngononophelo phambi kokwenza naluphi na uvavanyo.
★ Sukutya, usele okanye utshaye kwindawo apho imizekelo neekiti ziphathwa khona.Phatha yonke imizekelo ngokungathi inee-arhente ezosulelayo.Qwalasela izilumkiso ezimiselweyo malunga nobungozi be-microbiological kuyo yonke inkqubo kwaye ulandele iinkqubo ezisemgangathweni zokulahla ngokufanelekileyo iisampulu.Nxiba iimpahla ezikhuselayo ezifana needyasi zaselabhoratri, iiglavu ezilahlwayo kunye nokhuseleko lwamehlo xa imizekelo ivavanywa.
★ Musa ukutshintshiselana okanye udibanise ii-reagents ezivela kwiindawo ezahlukeneyo.
★ Ukufuma kunye nobushushu bunokuchaphazela kakubi iziphumo.
★ Izinto zokuvavanya ezisetyenzisiweyo kufuneka zilahlwe ngokuhambelana nemimiselo yendawo, urhulumente kunye / okanye i-federal.
Ukugcinwa kunye nokuzinza
✽ Ikhithi kufuneka igcinwe kwi-2-30°C de kube ngumhla wokuphelelwa kwexesha uprintwe kwisingxobo esivaliweyo.
✽ Uvavanyo maluhlale kwisingxobo esitywiniweyo de lusetyenziswe.
✽ Musa ukuba ngumkhenkce.
✽ Kufuneka kuthatyathwe inkathalo ukukhusela amalungu ale khithi ekosulelweni.Musa ukusebenzisa ukuba kukho ubungqina bokungcoliseka kwe-microbial okanye imvula.Ungcoliseko lwebhayoloji lwezixhobo zokukhupha, izikhongozeli okanye iirejenti kunokukhokelela kwiziphumo ezingezizo.
Ukuqokelelwa komfanekiso kunye noGcino
✔ IsiXhobo soVavanyo oluKhawulezayo lwe-HCV (iSerum/Plasma) senzelwe kuphela ukusetyenziswa neserum yabantu okanye iisampulu zeplasma.
✔ Kuphela imizekelo ecacileyo, engeyo-hemolyzed iyacetyiswa ukuba isetyenziswe kolu vavanyo.I-Serum okanye i-plasma kufuneka yahlulwe ngokukhawuleza kangangoko kunokwenzeka ukuphepha i-hemolysis.
✔ Yenza uvavanyo ngoko nangoko emva kokuqokelelwa komfanekiso.Musa ukushiya imizekelo kwiqondo lobushushu begumbi ixesha elide.Imizekelo ingagcinwa kwi-2-8°C ukuya kutsho kwiintsuku ezi-3.Ukugcina ixesha elide, imizekelo kufuneka igcinwe ngaphantsi kwe -20°C.
✔ Zisa iisampulu kwiqondo lobushushu legumbi ngaphambi kovavanyo.Iisampulu ezikhenkcezisiweyo kufuneka zinyibilike ngokupheleleyo kwaye zixutywe kakuhle phambi kovavanyo.Kuphephe ukukhenkceza okuphindaphindiweyo kunye nokunyibilika kwemizekelo.
✔ Pakisha imizekelo ngokuhambelana nemigaqo esebenzayo yokuthutha i-etiological agents, xa kufuneka zithunyelwe.
✔ I-icteric, i-lipemic, i-hemolysed, unyango lobushushu kunye ne-sera engcolileyo inokubangela iziphumo eziphosakeleyo.
Inkqubo
Zisa iimvavanyo, imizekelo, isithinteli kunye/okanye nolawulo kwiqondo lobushushu begumbi (15-30°C) phambi kokusetyenziswa.
1.Susa uvavanyo kwisingxobo salo esitywiniweyo, kwaye usibeke kwindawo ecocekileyo, esemgangathweni.Faka ileyibhile kwisixhobo ngesazisi sesigulane okanye solawulo.Ukufumana iziphumo ezingcono, uvavanyo kufuneka lwenziwe kwiyure enye.
2.Ukudlulisa i-2 yehla (malunga ne-50 L) ye-serum / i-plasma kunye ne-1 ye-drop buffer kwi-specimen well of device with a disposable pipette in the kit, and then start the timer.
Kuphephe ukubambisa amaqamza omoya kwiqula elingumzekelo (S), kwaye musa ukulahla nasiphi na isisombululo kwifestile yokuphonononga.
Njengoko uvavanyo luqala ukusebenza, uya kubona umbala uhamba kwi-membrane.
3.Lindela amabhanti anemibala avele.Isiphumo kufuneka sifundwe ngemizuzu eyi-10.Musa ukutolika umphumo emva kwemizuzu engama-20.
Ukutolikwa kweziPhumo
IZIPHUMO EZINYO: 
| * Ibhendi enemibala ibonakala kwingingqi yebhendi yolawulo (C) kwaye enye ibhendi enemibala ibonakala kwingingqi ye-T |
| IZIPHUMO EZINGAMBI:
| Ibhendi enye enemibala ibonakala kwingingqi yebhanti yolawulo (C).Akukho bhendi ivela kummandla webhendi yovavanyo (T). |
| IZIPHUMO EZINGAVUMI:
| Ibhendi yolawulo ayibonakali.Iziphumo zalo naluphi na uvavanyo olungakhange luvelise ibhendi yolawulo ngexesha lokufunda elichaziweyo mazichithwe.Nceda ujonge inkqubo kwaye uphinde ngovavanyo olutsha.Ukuba ingxaki iyaqhubeka, yeka ukusebenzisa ikhithi ngoko nangoko kwaye uqhagamshelane nomsasazi wakho wendawo |
Phawula
1. Ubunzulu bombala kwingingqi yovavanyo (T) bunokwahluka ngokuxhomekeka kugxininiso lwezinto ezijoliswe kuzo ezikhoyo kumzekelo.Ngoko ke, nawuphi na umthunzi wombala kummandla wovavanyo kufuneka uthathwe njengento enhle.Ngaphandle koko, inqanaba lezinto alinakumiselwa ngolu vavanyo lomgangatho.
2.I-specimen enganelanga umthamo, inkqubo yokusebenza engachanekanga, okanye ukwenza iimvavanyo eziphelelwe lixesha zizona zizathu ezinokwenzeka zokungaphumeleli kwebhendi yolawulo.
Ulawulo lwemeko
★ Ulawulo lwenkqubo lwangaphakathi lubandakanyiwe kuvavanyo.Ibhendi enemibala ebonakala kummandla wolawulo (C) ithathwa njengolawulo olulungileyo lwangaphakathi.Iqinisekisa umthamo owaneleyo wesampuli kunye nobuchule benkqubo obuchanekileyo.
★ Ulawulo lwangaphandle alubonelelwanga nale khithi.Kucetyiswa ukuba ulawulo oluqinisekileyo nolungalunganga luvavanywe njengenkqubo efanelekileyo yaselabhoratri yokuqinisekisa inkqubo yovavanyo kunye nokuqinisekisa ukusebenza kakuhle kovavanyo.
Imida yoVavanyo
1.I-HCV Rapid Test Device (iSerum/Plasma) yeyokusetyenziswa koxilongo lwe-in vitro yobungcali, kwaye kufuneka isetyenziselwe ukufumanisa umgangatho wezilwa-buhlungu ze-HCV kuphela.
2.I-HCV Rapid Test Device (iSerum/Plasma) iya kubonisa kuphela ubukho be-HCV antibodies kumzekelo kwaye mayingasetyenziswa njengeyona ndlela yodwa yokuxilongwa kosulelo lwentsholongwane ye-HCV.
3.Ukuba iziphumo zovavanyo zibi kwaye iimpawu zeklinikhi ziqhubeka, uvavanyo olongezelelweyo usebenzisa ezinye iindlela zeklinikhi ziyacetyiswa.Isiphumo esibi asitsho nangaliphi na ixesha ubukho bee-antibodies ze-HCV egazini, kuba izilwa-buhlungu zinokuthi zingabikho okanye zibe ngaphantsi komgangatho ophantsi wovavanyo.
4.Njengazo zonke iimvavanyo zokuxilonga, ukuxilongwa okuqinisekisiweyo kufuneka kwenziwe kuphela ngugqirha emva kokuba zonke iziphumo zeklinikhi kunye nebhubhoratri ziye zavavanywa.
Iimpawu zokuSebenza
Uluhlu: Uvavanyo oluKhawulezayo lwe-HCV vs. EIA
| Uvakalelo olunxulumeneyo:99.8% (99.0% -100.0%)* Iinkcukacha Ngokuhambelanayo:99.9% (99.8% -100.0%)* Isivumelwano sisonke:99.9% (99.7%-99.9%)* *95% iThutyana lokuzithemba | Uvavanyo olukhawulezayo lwe-HCV | ||||
| + | - | Iyonke | |||
| I-EIA | + | 565 | 2 | 567 | |
| - | 1 | 2543 | 2544 | ||
| 566 | 2545 | 3111 | |||
Iimbekiselo zoncwadi
1.Choo, QL, G. Kuo, AJ Weiner, LR Overby, DW Bradley, kunye noM. Houghton.Ukwahlukaniswa kwe-cDNA clone ethathwe kwi-genome yentsholongwane ye-hepatitis engeyiyo i-A, engeyiyo-B esegazini.Inzululwazi 1989;244:359
2.Kuo, G., QL Choo, HJ Alter, kunye noM. Houghton.Uvavanyo lokujikeleza izilwa-buhlungu ukuya kwiNtsholongwane ye-etiologic enkulu yabantu engeyiyo i-A, i-hepatitis engeyiyo ye-B.Inzululwazi 1989;244:362
I-3.van der Poel, i-CL, i-HTM Cuypers, i-HW Reesink, kunye ne-PNLelie.Ukuqinisekiswa kosulelo lweNtsholongwane ye-Hepatitis C ngovavanyo olutsha lwe-antigen ezine-recombinant immunoblot.iLancet 1991;337:317
4.Wilber, JC Uphuhliso kunye nokusetyenziswa kweemvavanyo zelabhoratri yokusuleleka kwe-hepatitis C: ukuhlaziywa.J. Clin.Uvavanyo lwe-Immunoassay 1993;16:204
